StressChron Framework:
| Project code | 010 |
| Acronym | CORTICAL |
| Role | Coordinator |
| Research area | Unravelling the fundamentals of glucocorticoid actions across vertebrates |
| Research topic | Mechanism(s) of glucocorticoid incorporation |
| Vertebrate | Fish |
| Species | Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), pike perch (Sander lucioperca), carp (Cyprinus carpio), Nile tilapia (Oreochromis niloticus), Mossambique tilapia (Oreochromis mossambicus), sole (Solea solea), zebrafish (Danio rerio) |
Abstract:
The research goal is to find a tissue in fish that incorporates glucocorticoids, in particular cortisol, in time hereby providing a retrospective view on the hypothalamic-pituitary-interrenal (HPI) axis making it the long-sought biomarker for chronic stress in fish. Hereto, an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for quantification of a glucocorticoid profile, as seen in Teleost fish, in calcified structures such as scales, otoliths, spines and soft fin ray of fish was developed in an EN ISO/IEC 17025 regulated environment and analytically validated according the requirements of the Commission Decision No. 2002/657/EC. Hereby, scales as well as, where applicable, other matrices such as otoliths, spines and soft fin ray from various species of Teleost fish were screened. Species tested included: Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), pike perch (Sander lucioperca), carp (Cyprinus carpio), Nile tilapia (Oreochromis niloticus), Mossambique tilapia (Oreochromis mossambicus), sole (Solea solea), and zebrafish (Danio rerio).
Specifics:
Keywords: aquaculture, fish, biomarker, stress, cortisol, scale
Funding: Internal
Status: Finalized
Consortium:
Ghent University, Belgium (Aerts J)
Flanders Research Institute for Agriculture, Fisheries and Food, Belgium (Aerts J)
Output:
Patent ‘Quantification of glucocorticoids in fish scales as biomarkers for chronic stress’ (EP3084432, US9638703, etc.)
Heymans K. Master thesis: Cortisol as biomarker for chronic stress across matrices and species. Faculty of Bioengineering Sciences, Hogeschool Ghent, Belgium, 2013.