StressChron Framework:
| Project code | 083 |
| Acronym | CORTSALAM |
| Role | Co-coordinator |
| Research area | Contributing to a more sustainable and animal welfare minded food production |
| Research topic | Improving our understanding of the impact of anthropogenic activities on the health status of vertebrates in the wild |
| Vertebrate | Amphibian |
| Species | Fire salamander (Salamandra salamandra) |
Abstract:
The research goal is to find a tissue in amphibians that incorporates glucocorticoids, in particular corticosterone, in time hereby providing a retrospective view on the hypothalamic-pituitary-adrenal (HPA) axis making it the long-sought biomarker for chronic stress in amphibians. Hereto, an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for quantification of a glucocorticoid profile, as seen in fire salamander (Salamandra salamandra), in various structures was developed in an EN ISO/IEC 17025 regulated environment and analytically validated according the requirements of the Commission Decision No. 2002/657/EC.
Specifics:
Keywords: wildlife, amphibian, biomarker, stress, UPLC-MS/MS, glucocorticoids
Funding: Internal
Status: On-going
Consortium:
Ghent University (Aerts J, Martel A, Pasmans F)
Flanders Research Institute for Agriculture, Fisheries and Food (Aerts J)
Output:
None at present