|Research area||Contributing to a more sustainable and animal welfare minded food production|
|Research topic||Improving our understanding of the impact of anthropogenic activities on the health status of vertebrates in the wild|
|Species||Fire salamander (Salamandra salamandra)|
The research goal is to find a tissue in amphibians that incorporates glucocorticoids, in particular corticosterone, in time hereby providing a retrospective view on the hypothalamic-pituitary-adrenal (HPA) axis making it the long-sought biomarker for chronic stress in amphibians. Hereto, an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for quantification of a glucocorticoid profile, as seen in fire salamander (Salamandra salamandra), in various structures was developed in an EN ISO/IEC 17025 regulated environment and analytically validated according the requirements of the Commission Decision No. 2002/657/EC.
Keywords: wildlife, amphibian, biomarker, stress, UPLC-MS/MS, glucocorticoids
Ghent University (Aerts J, Martel A, Pasmans F)
Flanders Research Institute for Agriculture, Fisheries and Food (Aerts J)
None at present