|Research area||Unravelling the impact of chronic stress across vertebrates|
|Research topic||Feed as stressor focusing on invertebrates (e.g. Artemia), mycotoxins and fish feed constituents|
|Species||Invertebrate Artemia franciscana (Great Salt Lake, USA)|
The research goal is to unravel whether Artemia, an invertebrate commercially used as exclusive feed in the larval stage of many Teleost fish in aquaculture, is a potential source of glucocorticoids, the stress hormones produced by vertebrates including fish, and can be a potential effector of the ontogeny of the hypothalamic-pituitary-interrenal (HPI) axis in fish larvae. Hereto, an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method for quantification of a glucocorticoid profile, as seen in Teleost fish, in Artemia was developed in an EN ISO/IEC 17025 regulated environment and analytically validated according the requirements of the Commission Decision No. 2002/657/EC. Hereby, focus was on Artemia franciscana (Great Salt Lake, USA).
Keywords: aquaculture, fish, larva, Artemia, stress, UPLC-MS/MS, glucocorticoids
Ghent University, Belgium (Aerts J)
Flanders Research Institute for Agriculture, Fisheries and Food, Belgium (Aerts J)
None at present