At StressChron we accommodate experiments throughout the life cycle of freshwater fish, from egg to adult, and develop experiments according to your needs. As a model organism, we focus on the common carp (Cyprinus carpio), one of the most widely cultivated and commercially important freshwater fish species in the world.
The experimental facilities at StressChron contain 51 individual and identical freshwater Recirculating Aquaculture Systems (RAS). There are three 700 L tanks, including one with an insert possibility to keep eggs and larvae, and two for own carp broodstock. Furthermore, there are two units each consisting of twelve 50 L tanks and another two units each consisting of twelve 100 L tanks. These custom-made facilities can thus accommodate broodstock, eggs, larvae, juvenile and adult fish. Each RAS is fully automated and equipped with a LED system, water renewal system, leakage detection, feed dispensers and online water quality monitoring system, ensuring full control of all parameters. In addition, relative humidity and temperature are controlled in each unit to guarantee a highly regulated environment.
Samples taken in this highly regulated environment are then analyzed in our laboratory facilities according to ISO 17025 standards. Besides our patented UPLC-MS/MS quantification methods for GC profiling in e.g. eggs, larvae and fish scales, a full gene expression profile of stress-related genes and a biochemical profile (osmolality, cytokines, glucose and lactate) are determined, while also elemental and structural analyses are performed on fish scales. This broad range of analyses allow for both in-depth fundamental and applied research on acute and chronic stress in fish mediated by glucocorticoids.